After being processing of the pre-mRNA is completed, the strand of mRNA is ready to be spliced. Splicing is the stage during which introns are removed from the strand, and the exons that remain are assembled into a finished strand of mRNA that is ready for translation. This section provides an animation and set of stills that cover the basic steps in mRNA splicing.After being transcribed, pre-mRNA must be processed. During processing certain features are added to the nascent mRNA. This section provides an animation and set of stills that cover the basic steps in mRNA processing.Basics of replication.

Semiconservative: after replication, each double stranded molecule is composed of an original strandAnd a newly copied strand.Two DNA strands separate.Each strand acts as a template for assembling a complimentary strand.Nucleotides line up singly along the template strand according to their base pairing rules.
Enzymes link the nucleotides together at their sugar phosphate groups.DNA replication is carried out by a large group of Enzymes. Origins of replication: species sites in the DNA molecule with specific base sequences.
Specific proteins required to initiate replication bind to each origin site.
The DNA helix opens and at the origins and a replication bubble forms.
Replication forks spread in each direction away from the initiation point.The PowerFood™ Microbial DNA Isolation Kit is designed to isolate high-quality genomic DNA from food cultured microorganisms.
The PowerFood™ Microbial DNA Isolation Kit stands out from other commercial food kits with its easy-to-use protocol that allows for isolation of DNA from a variety of food pathogens. Further, it is the only complete kit on the market, eliminating the need to purchase additional reagents and enzymes.
The PowerFood™ Microbial DNA Isolation Kit incorporates our patented Inhibitor Removal Technology® (IRT) to remove PCR-inhibiting compounds associated with food cultures, including lipids and polysaccharides. The kit’s buffers provide high quality, inhibitor-free DNA for use in downstream applications including PCR, qPCR and genotyping.
Food samples examined using the PowerFood™ Microbial DNA Isolation Kit include meats, cheeses and dairy products, chocolate, fruits, vegetables, and juices. Food pathogen DNA isolated from the following organisms was shown to be PCR inhibitor free: Clostridium difficile, Clostridium perfringens, Enterococcus faecalis, Escherichia coli, Lactobacillus sp., Listeria monocytogenes, and Salmonella enterica.