The UltraClean® Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.
Fresh or frozen plant tissues are homogenized using standard manual methods. After cell lysis, the released RNA is captured on a silica membrane spin filter. The RNA bound to the filter is washed to remove all contaminants. Finally, the RNA is recovered in certified RNase-free water (provided). The eluted RNA is very pure and ready for sensitive applications such as reverse-transcription PCR.The PowerPlant® DNA Isolation Kit isolates genomic DNA free from PCR impurities from even the toughest plant samples using a powerful homogenization method. Due to the unique hands-off bead beating method, up to 24 samples can be processed simultaneously using a vortex adapter or a bench top homogenizer, such as our Precellys® 24 Homogenizer. This method effectively eliminates both bottlenecks and cross-contamination risk found with hand grinding with a mortar and pestle.
The PowerPlant® DNA Isolation Kit saves significant time when compared to traditional liquid nitrogen methods and yields are comparable. In addition, its novel PCR inhibitor removal system ensures the DNA is free of polyphenols and polysaccharides allowing for all downstream applications including successful PCR analysis.
Powerful Protocol
The PowerPlant® DNA Isolation Kit protocol begins by adding plant material to the provided tubes containing specialized beads along with optimized reagents for rapid homogenization. Cell lysis and DNA release occur by both mechanical and chemical methods to maximize DNA isolation efficiency. A silica membrane in a spin column format is used to capture, wash and elute the pure DNA.
The PowerPlant® DNA Isolation Kit has been successfully utilized on a wide variety of plant types including leaf, roots, and seed samples. Occasionally, plants such as pine needles, cotton, sunflower, and strawberry present additional challenges and require an additional clean up procedure. The PowerPlant® DNA Isolation Kit provides special clean-up reagents as an optional step to assure success among even the most challenging plant samples.